Ticks are important ectoparasites of the livestock and wild animals worldwide. Ticks and tick-borne diseases are major problems in livestock health, especially in tropical and subtropical countries Ticks infection cause loss of blood and potentially deploy a variety of pathogenic agents (vectors) blood parasites such as Babesia and Anaplasma. Some of cattle ticks was   a complex of sibling species that are not easily distinguished based solely on morphological characters. This study aims to determine molecular character of cattle ticks   from Indonesia. The tick samples obtained from various regions in Indonesia. Females and males  were identified by using a stereo microscope. The ticks were identified as Rhipicephalus microplus and Rhipicephalus pilans. Molecular examination was conducted using samples at the larval stage. Their DNA was isolated and amplified in the region of the ribosomal DNA Internal trancribed pacer2 (ITS2) using PCR technique and finally it was sequenced. The results were then analyzed using Mega V. 6.0 program. The result sequences were aligned wof the allignment of multiple product sequencing ITS 2 gives sequences of nucleotides along in 1105 nucleotides. Genetic distance based on the sequence of the nucleotide sequence using Kimura-2 parameter revealed that the highest was 1.2%, the smallest was 0%. The comparisons of ITS2 sequences revealed a nucleotide similarity of 99-100% with each other and presented difference among the ITS2 sequences from Gene Bank of Boophilus species registered in GenBank. Neighbor's joining phylogenetic were constructed with   the ITS2 nucleotide sequence consisting of three clusters: the first group closely related to ticks China, the second group is closely related to ticks R. australis of Australia; The third group is related ticks of Laos, India and Cambodia origin. Identification of cattle ticks were successfully performed with ITS2.  R. microplus and R. pilans species could be distinguished from other tick species based on ITS2 nucleotide sequences.

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Angus, B. M. (1996). The history of the cattle tick Boophilus microptus in Australia and achievements in its control. International Journal for Parasitology, 26(12), 1341-1355.

Barker, S. C. 1998. Distinguishing species and populations of rhipicephaline ticks

with ITS 2 ribosomal RNA. J Parasitol, 84, 887-92.

Bickford D, Lohman DJ, Sodhi NS, Ng PKL, Meier R, Winker K, Ingram KK, Das I. Cryptic species as a window on diversity and conservation.2007. Trends Ecol Evol. 22:148–155

Cruickshank ,R, H. 2002. Molecular markers for the phylogenetics of mites and ticks Systematic and Applied Acarology .: 3-14

Dela Fuente, J., Estrada -Pena , A., Venzal, J. M., Kocan, K. M. , Sonenshine D.

E. 2008. Overview: Ticks as vectors of pathogens that cause disease in humans and animals. Front Biosci, 13, 6938-46.

Fukunaga, M., Yabuki, M., Hamase, A., Oliver, J. H., Jr., & Nakao, M. (2000). Molecular phylogenetic analysis of ixodid ticks based on the ribosomal DNA spacer, internal transcribed spacer 2, sequences. J Parasitol, 86(1), 38-43. doi: 10.1645/0022-3395(2000)086[0038:mpaoit];2

Peter R.J., Van den Bossche P., Penzhorn B.L., Sharp, B. 2005).Tick, fly, and mosquito control lessons from the past, solutions for the future. Vet Parasitol .132:205–215.

Roy, B. C., Estrada-Pena, A., Krucken, J., Rehman, A., & Nijhof, A. M. (2018). Morphological and phylogenetic analyses of Rhipicephalus microplus ticks from Bangladesh, Pakistan and Myanmar. Ticks Tick Borne Dis, 9(5), 1069-1079. doi: 10.1016/j.ttbdis.2018.03.035

Tamura, K., Stecher, G., Peterson, D., ilipsiI, A.and Kumar , S. 2013. MEGA6:

molecular evolutionary genetics analysis version 6.0. Molecular biology and evolution, 30, 2725-2729.


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